Class III viral membrane fusion proteins
نویسندگان
چکیده
منابع مشابه
Negative Potentials Across Biological Membranes Promote Fusion by Class II and Class III Viral Proteins
Voltage was investigated as a factor in the fusion of virions. Virions, pseudotyped with a class II, SFV E1 or VEEV E, or a class III protein, VSV G, were prepared with GFP within the core and a fluorescent lipid. This allowed both hemifusion and fusion to be monitored. Voltage clamping the target cell showed that fusion is promoted by a negative potential and hindered by a positive potential. ...
متن کاملViral membrane fusion: is glycoprotein G of rhabdoviruses a representative of a new class of viral fusion proteins?
Enveloped viruses always gain entry into the cytoplasm by fusion of their lipid envelope with a cell membrane. Some enveloped viruses fuse directly with the host cell plasma membrane after virus binding to the cell receptor. Other enveloped viruses enter the cells by the endocytic pathway, and fusion depends on the acidification of the endosomal compartment. In both cases, virus-induced membran...
متن کاملDomain III from class II fusion proteins functions as a dominant-negative inhibitor of virus membrane fusion
Alphaviruses and flaviviruses infect cells through low pH-dependent membrane fusion reactions mediated by their structurally similar viral fusion proteins. During fusion, these class II viral fusion proteins trimerize and refold to form hairpin-like structures, with the domain III and stem regions folded back toward the target membrane-inserted fusion peptides. We demonstrate that exogenous dom...
متن کاملThe Transmembrane Domain and Acidic Lipid Flip-Flop Regulates Voltage-Dependent Fusion Mediated by Class II and III Viral Proteins
Voltage dependence of fusion induced by class II and class III viral fusion proteins was investigated. Class II proteins from Ross River and Sindbus virus and a mutant class III protein from Epstein Barr virus were found to induce cell-cell fusion that is voltage dependent. Combined with previous studies, in all, four class II and two class III protein have now been shown to exhibit voltage-dep...
متن کاملDifferential cholesterol binding by class II fusion proteins determines membrane fusion properties.
The class II fusion proteins of the alphaviruses and flaviviruses mediate virus infection by driving the fusion of the virus membrane with that of the cell. These fusion proteins are triggered by low pH, and their structures are strikingly similar in both the prefusion dimer and the postfusion homotrimer conformations. Here we have compared cholesterol interactions during membrane fusion by the...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2009
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2009.02.012